一体式数字显微镜

Invitrogen EVOS FL荧光细胞成像仪

Invitrogen EVOS FL荧光细胞成像仪带有明视场通道和4个荧光通道,可用于观察、采集和处理细胞样品图像,具有自动细胞计数,延时拍摄功能,适用于培养活细胞生长状态观察和记录。

设备名称:细胞荧光成像仪 Digital Fluorescence Microscope

制 造 商:美国赛默飞(Thermo Fisher)公司   产地:新加坡

品    牌:英捷(Invitrogen)

型    号:EVOS FL(discontinued

主要功能用途

Invitrogen EVOS FL荧光细胞成像仪带有明视场通道和4个荧光通道,是一个完整的数字成像系统,可用于观察样品,采集和存储图像,并进行图像多色叠加处理。此外,EVOS FL荧光细胞成像仪具有自动细胞计数,延时拍摄功能。适合日常的细胞培养工作及荧光应用,比如细胞融合度的预测,细胞形态的观察,细胞生长和增殖监控,细胞图像的采集(有荧光或无荧光),荧光蛋白表达的观察以及转染效率的预测。

 

性能特点

Invitrogen EVOS FL荧光细胞成像仪一体化设计采用15寸大屏幕LCD显示器取代传统的目镜直接进行目视观察,角度可调,符合人体工程学,舒适方便,视野开阔;可以整体放入到超净台或生物安全柜内,方便各种细胞实验操作。

EVOS系列产品去除了传统显微镜的复杂操作,将倒置显微镜的常规基本功能融为一体,只需轻轻一点即可获得高品质图像。

Time lapse功能:可对运动的样品进行实时动态拍摄,保留图像并进行动画制作;

细胞计数功能:预设全自动计数程序,可自动定量非荧光标记细胞、可同时对两种不同荧光通道的细胞进行计数,可以提供细胞总浓度、活细胞和死细胞所占比例和细胞显微图片;

LED光源温度稳定、产热极少,可达5万小时使用无需维护,无需更换灯泡和保险丝。

所有EVOS 荧光细胞成像系统都采用LED光源,包括了DAPI、GFP、RFP、CFP、YFP、Texas Red、Cy5、 Cy7等多种常规荧光模块及10种量子点模块可选;专利的LED光立方可精确的强度调整,减少光漂白。

 

主要技术规格

EVOS FL荧光细胞成像仪分EVOS FL、EVOS FL Color两个版本,主要技术区别在其预装单色CCD、彩色CCD之不同。

型号

EVOS FL

EVOS FL Color

光学系统

无限校准光学系统;45mm等焦距离的RMS螺纹物镜

物镜类型

多种类型物镜可选

物镜转盘

5位转盘,前置控制

聚光镜

包括透射光源,聚光镜,相差环等光学组件

聚光器工作距离

60 mm

照明系统

强度可调的LED光源,使用寿命大于50000小时

对比方法

落射荧光与透射光(明场及相差)

荧光通道

同时可容纳达4荧光立方

荧光光立方

有20种通用荧光染料通道供选,常用三种荧光立方:

DAPI(Ex/360 nm,   Em/447 nm);GFP(Ex/470   nm,  Em/525 nm);RFP(Ex/530   nm,  Em/593 nm)

载物台

X-Y轴定位控制机械滑动载物台 ,110x110mm移动范围,可固定

X、Y定位控制精度:28.3mm/圈;Z轴聚焦480μm/圈

有多种容器支架适配框可选

可支持的样品容器

培养皿、培养瓶、微孔板以及血球计数板、玻片

LCD显示屏

15”高分辨率(1024×768像素)彩色显示器,倾斜度可调

照相机

Sony ICX445单色CCD:

1/3” 尺寸,130万像素

Sony ICX285AQ彩色半导体制冷CCD:

2/3” 尺寸,140万像素

图像格式

16位单色TIFF或PNG (12 bit 动态范围);24 bit 彩色TIFF 或PNG,JPEG,BMP格式

输出端口

3个USB端口,1个DVI端口

 

安装运行条件

Invitrogen EVOS FL荧光细胞成像仪只能在室内使用

工作环境温度:4℃ ~ 32℃

工作电源:AC 100-240V AC, 50/60Hz; Output 12V DC/4.15A

相对湿度范围:30% ~ 90%

主机重量:15.3 kg

外观尺寸尺寸(W×D×H):355×470×578 mm

 

组成配置

Invitrogen EVOS FL荧光细胞成像工作组件由主机(含数码相机、聚光镜遮光罩Condenser shield)、物镜系统、荧光光立方(Fluorescent light cube)和配套工具、样品容器适配器(支架)和辅助工具套件组成。工具包包括鼠标,Block Sliders,Light shield box,UV shield assembly, Light cube access cover。

针对不同实验应用和样品容器,需选配响应物镜、光立方、样品容器适配器和聚光镜光学插件(滑块)。

1、细胞荧光成像可选物镜

品名

品牌

货号

规格

描述

报价

4X长距离消色差相差物镜

Invitrogen

AMEP4632

Each

EVOS   4X Objective, achromat, LWD, phase-contrast

Magnification:4X

Numerical   Aperture:0.13

Working   Distance:16.9 mm

询价

4X平场荧光物镜

Invitrogen

AMEP4622

Each

EVOS   4X Objective, fluorite, LWD

Magnification:4X

Numerical   Aperture:0.13

Working   Distance:19.7 mm

询价

4X平场荧光相差物镜

Invitrogen

AMEP4980

each

EVOS 4X Objective, fluorite, LWD, phase-contrast, 0.13NA/10.58WD

Magnification:4X

Numerical   Aperture:0.13

Working   Distance:10.58 mm

询价

4X平场荧光相差物镜

Invitrogen

AMEP4680

each

EVOS   4X Objective, fluorite, LWD, phase-contras

Magnification:4X

Numerical   Aperture:0.13

Working   Distance:16.9 mm

询价

10x平场荧光相差物镜

Invitrogen

AMEP4981

each

EVOS 10X Objective, fluorite, LWD, phase-contrast, 0.30NA/7.13WD

Magnification:   10X

Numerical   Aperture: 0.30

Working   Distance: 7.13 mm

询价

10x平场荧光相差物镜

Invitrogen

AMEP4681

each

EVOS   10X Objective, fluorite, LWD, phase-contrast

Magnification:10X

Numerical   Aperture:0.25

Working   Distance:9.2 mm

询价

10x平场荧光物镜

Invitrogen

AMEP4623

Each

EVOS   10X Objective, fluorite, LWD

Magnification:10X

Numerical   Aperture:0.30

Working   Distance:8.3 mm

询价

20x平场荧光物镜

Invitrogen

AMEP4624

Each

EVOS   20X Objective, fluorite, LWD

Magnification:20X

Numerical   Aperture:0.45

Working   Distance:7.1 mm

询价

20x平场荧光物镜

Invitrogen

AMEP4698

each

EVOS   20X Objective, fluorite, coverslip-corrected

Magnification:20X

Numerical   Aperture:0.50

Working   Distance:2.5 mm

询价

20x平场荧光相差物镜

Invitrogen

AMEP4982

each

EVOS 20X Objective, fluorite, LWD, phase-contrast, 0.45NA/6.12WD

Magnification:   20X

Numerical   Aperture: 0.45

Working   Distance: 6.12 mm

询价

40x平场荧光物镜

Invitrogen

AMEP4625

each

EVOS   40X Objective, fluorite, LWD

Magnification:40X

Numerical   Aperture:0.65

Working   Distance:2.8 mm

询价

40x平场荧光物镜

Invitrogen

AMEP4925

each

EVOS 40X Objective, fluorite, LWD, 0.65NA/1.79WD

Magnification:40X

Numerical   Aperture:0.65

Working   Distance:1.79 mm

询价

40x平场荧光相差物镜

Invitrogen

AMEP4983

each

EVOS 40X Objective, fluorite, LWD, phase-contrast

Magnification:40X

Numerical   Aperture:0.65

Working   Distance:1.79 mm

询价

40x平场荧光相差物镜

Invitrogen

AMEP4683

each

EVOS   40X Objective, fluorite, LWD, phase-contrast

Magnification:40X

Numerical   Aperture:0.65

Working   Distance:1.6 mm

询价

40x带校准平场荧光物镜

Invitrogen

AMEP4699

each

EVOS   40X Objective, fluorite, coverslip-corrected

Magnification:40X

Numerical   Aperture:0.75

Working   Distance:0.72 mm

询价

100X 带校准平场荧光物镜(油镜)

Invitrogen

AMEP4696

each

Invitrogen   EVOS 100X Oil Objective, fluorite, coverslip-corrected

Magnification:   100X

Numerical   Aperture: 1.28

Working   Distance: 0.21 mm

Immersion:   oil

询价

 

2、常用荧光光立方选项

名称

品牌

货号

规格

描述

报价

DAPI 荧光光立方

Invitrogen

AMEP4650

each

EVOS DAPI Fluorescent light cube

Ex: 357/44 nm,Em: 447/60 nm

Compatible Dyes: DAPI,Hoechst,BFP

询价

询价

GFP荧光光立方

Invitrogen

AMEP4651

each

EVOS GFP Fluorescent light cube

Ex:  470/22 nm,Em: 510/42 nm

Compatible Dyes: GFP,Alexa Fluor 488,SYBR Green,FITC

询价

询价

RFP荧光光立方

Invitrogen

AMEP4652

each

EVOS RFP Fluorescent light cube

Ex:  531/40 nm,Em: 593/40 nm

Compatible Dyes:

RFP/Alexa Fluor 546/Alexa   Fluor 555/Cy3/DsRed/Rhodamine Red/dTomato

询价

询价

CFP荧光光立方

Invitrogen

AMEP4653

each

CFP Fluorescent Light Cube

Ex: 445/45nm Em: 510/42 nm

Compatible Dyes: ECFP,Lucifer Yellow

询价

询价

YFP 荧光光立方

Invitrogen

AMEP4654

each

EVOS YFP   Fluorescent light cube

Ex:  500/24 nm,Em: 524/27 nm

Compatible Dyes: EYFP

询价

询价

Texas Red 荧光光立方

Invitrogen

AMEP4655

each

EVOS Texas red   Fluorescent light cube

Ex: 585/29   nm,Em:   624/40 nm

Dyes: Texas Red/Alexa Fluor 568/Alexa Fluor 594/MitoTracker Red/mCherry

询价

询价

Cy5 荧光光立方

Invitrogen

AMEP4656

each

EVOS CY5   Fluorescent light cube

Ex: 628/40   nm,Em:   692/40 nm

Compatible Dyes: Cy5,Alexa Fluor 647,Alexa Fluor 660,DRAQ5

询价

询价

Qdot 525 荧光光立方

Invitrogen

AMEP4657

each

EVOS Qdot 525   light cube

Ex: 445/45   nm,Em:   525/15 nm

询价

Qdot 545 荧光光立方

Invitrogen

AMEP4658

each

EVOS Qdot 545   light cube

Ex: 445/45   nm,Em:   544/24 nm

询价

Qdot 565 荧光光立方

Invitrogen

AMEP4659

each

EVOS Qdot LED   Light Cubes

Ex: 445/45   nm,Em:   567/15 nm

询价

Qdot 585 荧光光立方

Invitrogen

AMEP4660

each

EVOS Qdot 585   LED Light Cubes

Ex: 445/45   nm,Em:   588/20 nm

询价

Qdot 605 荧光光立方

Invitrogen

AMEP4661

each

Qdot 605 light   cube

Ex: 445/45   nm,Em:   605/20 nm

Compatible Dyes: Qdot 605 and other fluorophores

询价

Cy7 荧光光立方

Invitrogen

AMEP4667

each

Cy7 Fluorescent   light Cubes

Ex: 710/40   nm,Em:   809/81 nm;

询价

TagBFP 荧光光立方

Invitrogen

AMEP4668

each

TagBFP Fluorescent light Cubes

Ex: 390/18nm,Em: 447/60nm

Dyes: CascadeBlue,Pacific   Blue,TagBFP   and other fluorophores

询价

 

3)可选样品固定器

 

品名

品牌

货号

规格

描述

报价

Corning   T-25培养瓶2联适配器

Invitrogen

AMEPVH005

each

EVOS Vessel Holder, two 25-cm T-flasks

询价

Falcon/Greiner   T-25培养瓶适配器

Invitrogen

AMEPVH010

each

EVOS Vessel Holder, Greiner/BD T-25 flask

询价

Nunc   T-25培养瓶适配器

Invitrogen

AMEPVH018

each

EVOS Vessel Holder, one Nunc T-25 flask

询价

Nunc   T-75培养瓶适配器

Invitrogen

AMEPVH006

each

EVOS Vessel Holder, Nunc T-75 flask

询价

Greiner   T-75培养瓶适配器

Invitrogen

AMEPVH008

each

EVOS Vessel Holder, Greiner T-75 flask

询价

SPL T-75培养瓶适配器

Invitrogen

AMEPVH012

each

EVOS Vessel Holder, SPL T-75 flask

询价

Nunc   60mm培养皿2联适配器

Invitrogen

AMEPVH003

each

EVOS Vessel Holder, two 60-mm Petri dishes

询价

35mm培养皿4联适配器

Invitrogen

AMEPVH002

each

EVOS  Vessel Holder, four 35-mm Petri dishes

询价

Nunc   100mm培养皿适配器

Invitrogen

AMEPVH004

each

EVOS Vessel Holder, one 100-mm Petri dish

询价

IBIDI 50 mm培养皿2联适配器

Invitrogen

AMEPVH014

each

EVOS Vessel Holder, ibidi 50 mm Petri dishes

询价

IBIDI 35 mm培养皿2联适配器

Invitrogen

AMEPVH013

each

EVOS Vessel Holder, ibidi 35 mm Petri dishes

询价

培养板适配器

Invitrogen

AMEPVH011

each

EVOS Vessel Holder, Nunc/SPL IVF 4-well dish

 (L×W):66.5×66.5mm

询价

玻片适配器

Invitrogen

AMEPVH019

each

EVOS   Vessel Holder for Oil Objectives

one   25 x 75 mm microscope slide.

询价

玻片2联适配器

Invitrogen

AMEPVH001

each

EVOS   Vessel Holder, two 25 x 75 mm slides

询价

血球计数板适配器

Invitrogen

AMEPVH007

each

Hemocytometer   Holder

询价

Kova玻片适配器

Invitrogen

AMEPVH017

each

EVOS   Vessel Holder

accommodates one KOVA Glasstic Slide 10.

询价

样品容器适配器套装

Invitrogen

AMEP4619

each

EVOS   Vessel Holder Pack

Including:

25×75mm   Slide Holder(AMEPVH001)

35mm   Petri-Dish Holder(AMEPVH002)

60mm   Petri-Dish Holder(AMEPVH003)

100mm   Petri-Dish Holder(AMEPVH004)

T-25   Flask Holder(AMEPVH005)

Nunc   T-75 Flask Holder(AMEPVH006)

Hemocytometer   Holder(AMEPVH007)

Greiner   T-75 Flask Holder(AMEPVH008)

Universal   Holder(AMEPVH009)

Greiner/BD   T-25 Flask Holder(AMEPVH010)

询价

多孔板/T-75培养瓶托盘

Invitrogen

AMEP4686

each

Stage   plate for multi-well vessels;

also   hold one Corning T-75 flask

询价


多孔板托盘

Invitrogen

AMEP4687

each

Stage plate with 110 mm x 160 mm opening

(Use with AMEP4692 for standard sizes)

询价


EVOS   通用托盘

Invitrogen

AMEPVH065

each

Universal Stage Plate With a 1.5-inch opening

询价  


载物台功能拓展托盘

Invitrogen

AMEPVH048

each

Stage plate for automated stage vessel holders

询价


带25.4mm圆孔适配器

Invitrogen

AMEPVH009

each

universal Vessel Holder with 25.4 mm opening

询价


110×160   mm开孔托盘

Invitrogen

AMEP4691

each

Stage Plate with a 160 x 110 mm opening

询价


AMEP4691托盘用适配器

Invitrogen

AMEP4692

each

Staging   Plate with 160 X 110 mm opening

询价


带加热功能托盘

Invitrogen

AMEP4685

each

Stage Plate for BioFlux heating stage (Fluxion).

询价


方形托盘

Invitrogen

AMEPVP01

each

Vessel Plate for Large Vessels

询价


 

4、其它辅助附件

所有 EVOS 成像系统主机基座配置有1个DVI显示输出端口,用于将采集的图像输出到投影仪或其他显示器(不含DVI 电缆,需另配),便于工作小组共享、讨论。此端口与 DVI-D 或 DVI-I 显示屏兼容。视频传输速率可达每秒30帧@1920 x 1200 像素分辨率。

EVOS 臂部休息旨在帮助提高精度和减少长时间操作(如细胞分离和组织培养)期间的疲劳,可安装于仪器任意一侧。

品名

品牌

货号

规格

描述

单价

外接DVI视频采集卡

Invitrogen

AMEP4717

each

DVI-to-USB frame grabber

capture resolutions up to 1920 x 1200   pixels @30 fps.

询价

臂部休息套件

Invitrogen

AMEP4618

each

EVOS Arm Rest Kit fits on either side of   the instrument stage

the non-skid surface helps prevent   slipping.

询价

 

 

(略)

 

订货指南

Invitrogen EVOS FL荧光细胞成像(已下架)