小型蛋白垂直电泳槽

伯乐Mini-Protean Tetra蛋白电泳槽

伯乐Mini-Protean Tetra小型蛋白电泳槽可容纳1~4块小型手灌胶或Ready Gel 预制胶工作,用于蛋白SDS-PAGE垂直电泳分离。本栏目提供Mini-Protean Tetra的组成及选型配置方案。

设备名称:蛋白垂直电泳槽  蛋白垂直电泳仪
制 造 商:美国Bio-Rad公司           产 地:新加坡/中国
品    牌:伯乐(Bio-Rad)    
型    号:Mini-Protean Tetra
电泳配件:见[主要附件]  商品价格:见[订货指南]

主要功能用途

Bio-Rad Mini- Protean Tetra蛋白垂直电泳槽,是Western Blot实验流程重要组成部件,可容纳1~4 块小型手灌胶或Ready Gel 预制胶(10×8.3cm)的电泳,主要用于蛋白聚丙烯酰胺凝胶电泳(PAGE或SDS-PAGE)实验。

 

性能特点

伯乐Mini-Protean Tetra蛋白垂直电泳槽,兼容Mini-Protean precast gels预制凝胶、Ready Gel precast gels预制凝胶或手工自制PAGE凝胶。

可在一小时内完成多达四块小型蛋白凝胶的电泳分离。

软橡胶垫片与玻璃板形成严密密封,确保无泄漏灌胶。

带有简单凸轮的灌制框,确保在任何平面上实现精确对齐。

平行排列的灌胶架,能同时灌制两块凝胶,弹簧杠杆设计使得软橡胶沉淀产生良好的密封性。

封边垫条的长玻板加厚,不易破碎。

标有厚度和孔数的玻板和加样梳便于识别。

上样向导防止泳道遗漏上样或重复上样。

可互换模块可将Mini- Protean Tetra电泳槽转换为可进行Western转印的 Mini Trans-Blot转印槽。

 

 

主要技术规格

伯乐Mini-Protean Tetra蛋白垂直电泳槽适用的凝胶类型包括:Mini- Protean预制胶、手灌胶

凝胶数:1–4块

凝胶格式:小型

适用的凝胶尺寸(宽×长)

预制胶:8.6×6.8cm;

手灌胶:8.3×7.3cm

玻璃板尺寸:短板10.1×7.3cm,玻板10.1×8.2cm

缓冲液运行需求

运行2块凝胶缓冲液总体积:700mL

运行4块凝胶缓冲液总体积:1000 mL

SDS-PAGE 典型运行时间:35–45 分钟(在200V恒定电压下)

Mini-PROTEAN Tetra加样梳每孔最大上样量

1.0mm 10孔梳:44 μL

1.0mm 15孔梳:26 μL

 

安装运行条件

相对湿度范围:0~95% R.H.

工作环境温度:0℃~40℃

外观尺寸(宽×长×高):12×16×18cm

Mini- Protean Tetra 电泳槽重量:1.0kg

(电泳电源另配)

 

组成配置

电泳槽系统

伯乐Mini-Protean Tetra蛋白电泳槽全套工作装置包括电泳缓冲液槽电极组件制胶组件辅助工具耗材。
按一次可运行的最大凝胶数量,分为4-gel、2-gel两种规格。
如需跑4块凝胶,可订购4凝胶槽,也可在2凝胶套装基础上加配Mini-Protean Tetra相应功能附件,扩展为4块凝胶工作通量。

此外,根据使用的凝胶来源类型,还可单独配置伯乐预制胶专用套装。

品名

品牌

货号

规格

描述

报价

蛋白垂直电泳槽

(4-gel/10-well/1.0mm)

Bio-Rad

1658001

1套

Mini-PROTEAN Tetra Cell

4-gel system, 1.0mm gel thickness

includes:

Tank, lid with power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 1.0mm combs

5×gel releasers(1653320)

询价

询价

蛋白垂直电泳槽

(4-gel/10-well/1.5mm)

Bio-Rad

1658006

1套

Mini-PROTEAN Tetra Cell

4-gel system, 1.5mm gel thickness

includes:

Tank, lid, power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 1.5mm combs

5×gel releasers(1653320)

询价

询价

蛋白垂直电泳槽

(4-gel/10-well/0.75mm)

Bio-Rad

1658000

1套

Mini-PROTEAN Tetra Cell

4-gel system, 0.75mm gel thickness

includes:

Tank, lid, power cables

Mini cell buffer dam;

1×electrode assembly;

1×companion running module(1658038);

2×casting stands, 4 casting   frames;

5 sets of short plates and spacer plates;

5×10-well 0.75mm combs

5×gel releasers(1653320)

询价

询价

蛋白垂直电泳槽l

(2-gel/10-well/1.0mm)

Bio-Rad

1658003

1套

Mini-PROTEAN Tetra Cell

2-gel system, 1.0mm gel thickness

includes:

1×casting stand, 2×casting frames;

5×10-well combs;

5 short plates and 5 spacer plates

询价

询价

蛋白垂直电泳槽

(2-gel/10-well/1.5mm)

Bio-Rad

1658007

1套

Mini-PROTEAN Tetra Cell

2-gel system, 1.5mm gel thickness

includes:

1×casting stand, 2×casting frames;

5×10-well combs;

5 sets of glass plates

询价

询价

蛋白垂直电泳槽

(2-gel/10-well/0.75mm)

Bio-Rad

1658002

1套

Mini-PROTEAN Tetra Cell

2-gel system, 0.75 mm gel thickness

includes:

1×casting stand, 2×casting   frames;

5×10-well combs;

5 short plates and 5 spacer plates

询价

询价

Mini Precast Gels(4-gel)

预制胶蛋白电泳槽

Bio-Rad

1658004

1套

Mini-PROTEAN Tetra Vertical Electrophoresis Cell

for Mini Precast Gels, 4-gel

includes:

electrode assembly;

companion running module;

tank, lid with power cables, cell buffer   dam

询价

询价

Mini Precast Gels (2-gel)

预制胶蛋白电泳槽

Bio-Rad

1658005

1套

Mini-PROTEAN Tetra Vertical Electrophoresis  Cell

for Mini Precast Gels, 2-gel

includes:

electrode assembly;

tank, lid with power cables, cell buffer dam

询价

询价



电泳槽组件

伯乐Mini-Protean Tetra 小型蛋白垂直电泳槽的制胶模块(Casting Module),是指用于灌制特定厚薄和齿孔数目蛋白凝胶所需的模块化套装。

模块由通用核心组件(Core System)和差异化功能组件组成。通用核心灌胶组件为灌胶架(含4个密封垫),夹胶框(casting frames)和或胶铲(gel releaser)。差异化功能组件则包括特定规格的玻璃板套件,电泳梳和上样导槽。

伯乐Mini-PROTEAN Tetra Cell小型垂直电泳槽有多种灌胶模块可选,分别满足不同上样量和对凝胶厚度的特殊要求。

品名

品牌

货号

规格

描述

报价

1.0mm/10孔 制胶模块

Bio-Rad

1658015

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well/1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

询价

询价

1.0mm/15孔制胶模块

Bio-Rad

1658016

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well, 1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

询价

询价

1.0mm/5孔 制胶模块

Bio-Rad

1658013

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates.

询价

询价

1.0mm/9孔 制胶模块

Bio-Rad

1658014

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   1.0 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide.

询价

询价

1.5mm/10孔 制胶模块

Bio-Rad

1658021

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well, 1.5 mm gel casting module

includes:

2×casting stands; 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

询价

询价

1.5mm/15孔 制胶模块

Bio-Rad

1658022

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well, 1.5 mm gel casting module

includes:

2×casting stands, 4×casting frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

询价

询价

1.5mm/5孔 制胶模块

Bio-Rad

1658019

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   1.5 mm gel casting module

includes   the following componets:

2   casting stands, 4 casting frames

5   combs, 5 short plates and 5 spacer plates.

询价

询价

1.5mm/9孔 制胶模块

Bio-Rad

1658020

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   1.5 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

询价

询价

0.75mm/10孔 制胶模块

Bio-Rad

1658010

1套

Mini-PROTEAN Tetra Cell Casting Module

10-well,   0.75 mm gel casting module

includes   the following componets:

2   casting stands, 4 casting frames

5   combs, 5 short plates and 5 spacer plates

sample   loading guide.

询价

询价

0.75mm/15孔 制胶模块

Bio-Rad

1658011

1套

Mini-PROTEAN Tetra Cell Casting Module

15-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

询价

询价

0.75mm/5孔 制胶模块

Bio-Rad

1658008

1套

Mini-PROTEAN Tetra Cell Casting Module

5-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short   plates and 5 spacer plates;

1×sample loading guide

询价

询价

0.75mm/9孔 制胶模块

Bio-Rad

1658009

1套

Mini-PROTEAN Tetra Cell Casting Module

9-well,   0.75 mm gel casting module

includes:

2×casting stands, 4×casting   frames;

5×combs, 5 short plates and 5 spacer plates;

1×sample loading guide

询价

询价

灌胶通用套件(4胶)

Bio-Rad

1658051

1套

Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

includes:

2×stands, 4×frames,4×gaskets;

5×gel   releasers.

适用于4胶Mini-Protean Tetra电泳槽

询价

询价

灌胶通用套件(2胶)

Bio-Rad

1658052

1套

Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit

includes:

1×stand, 2×frames, 2×gaskets;

5×gel releasers.

适用于2胶Mini-Protean Tetra电泳槽

询价

询价

基本耗材

伯乐Mini-Protean Tetra垂直电泳槽工作的基本耗材主要包括蛋白凝胶玻璃板等。

品名

品牌

货号

规格

描述

报价

1.0mm厚玻璃板

Bio-Rad

1653311

5块/包

Spacer Plates with 1.0 mm Integrated Spacers

带1.0mm厚封边条,为1.0mm凝胶灌胶组件。

询价

1.5mm厚玻璃板

Bio-Rad

1653312

5块/包

spacer plate   with 1.5 mm integrated spacer

带1.5mm厚封边条,用于1.5mm凝胶的制备

询价

薄玻璃板

Bio-Rad

1653308

5块/包

short plate for Mini-PROTEAN Tetra System

 and Mini-PROTEAN 3 Multi-Casting Chamber.

外形尺寸(W×L):101× 73 mm

与带任一厚度封边垫条的长玻璃板配套使用

询价

 

订货指南

1、蛋白电泳槽的选型

因不同实验样品样品数量、蛋白类型和表达丰度、实验经验与操作习惯的不同,选择蛋白电泳槽重点要结合所用凝胶的厚度(0.75mm、1.0mm、1.5mm)、每张凝胶电泳的上样孔数(常用的10孔-最大上样量66μl、15孔-40μl)、每轮电泳的凝胶通量(4胶、2胶)和凝胶类型(手灌胶、预制胶)来确定具体选型方案。

如单独订购Mini-Protean Tetra小型蛋白垂直电泳槽或更换电泳灌胶配件,请请参考本栏目“组成配置”“主机”部分所列各型电泳槽的技术规格和详细配置内容。最常用的1658001槽单元,其标准配置适用的是1.0mm/10-well/4-gel凝胶。

 

2、蛋白电泳槽电源的选择

伯乐Mini-Protean Tetra小型蛋白垂直电泳槽本身工作运行对电源并无特殊要求。伯乐PowerPac Basic 、PowerPac HC、PowerPac Universal等多个型号均可满足同时给1-4个电泳槽供电的要求。

但须注意的是,通常情况下,蛋白电泳槽只是Western Blot实验流程的一个功能组件,单个电泳槽输出凝胶数量(2块或4块)与电转印槽(2块)各自最大凝胶工作通量不同,要充分考虑实验流程上的有效衔接问题。

特别是当实验样品数量多于4块凝胶的情况下,根据自身实验条件和经验,通盘考虑电转印槽、电转印模块的配置数量。

与常规140V左右恒压蛋白电泳跑胶不同,电转印对电流要求较高。通常,每块凝胶-印迹膜的工作电流可达200mA甚至更高。若同时进行4块凝胶转膜操作,单一Basic电源很可能无法满足供电要求。故需考虑HC、PowerPac Universal等具有电流输出能力大于0.8A的电源型号确有必要。

 

3、电泳槽与与Mini-Trans Blot小型蛋白电泳转印槽搭配

电转移槽的选型配置问题,请参考Mini-Trans Blot小型蛋白转印槽栏目内容。


序号

品名

货号

套装配置描述

报价

1-1

Basic蛋白电泳套装

(1.0mm/10-well/4-gel)

1658025

Mini-PROTEAN Tetra and Power Supply Bundle

includes:

Mini-PROTEAN Tetra 4-gel system (1658001);

PowerPac Basic power supply (1645050).

询价

询价

1-2

HC蛋白电泳套装

(1.0mm/10-well/4-gel)

1658027

Mini-PROTEAN Tetra and Power Supply Bundle

includes:

Mini-PROTEAN Tetra 4-gel system (1658001);

PowerPac HC power supply (1645052).

询价

询价






2-1

电泳-转印槽组合装

(1.0mm/10-well/4-gel/2-blot)

1658029

Electrophoresis and blot Bundle (10-well/1.0mm/4-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658001);

Mini Trans-Blot Module (1703935).

询价

询价

2-2

Basic电源-电泳-转印套装

(1.0mm/10-well/4-gel/2-blot)

1658033

Electrophoresis, blot and Power Supply   Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

询价

询价

2-3

HC电源-电泳-转印套装

(1.0mm/10-well/4-gel/2-blot)

1658035

Electrophoresis, blot and Power Supply Bundle

for 10-well, 1.0 mm, 4-gel system

includes:

Mini-PROTEAN Tetra Cell (1658001);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052)

询价

询价

2-4

HC电源-电泳-转印套装

(1.0mm/10-well/4-gel/4-blot)

1658035-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini Trans-Blot Transfer Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

询价

询价

2-5

HC电源-电泳-转印高级套装

(1.0mm/10-well/4-gel)

(1.5mm/10-well/4-gel)

(4-blot)

1658035-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001);

Mini-PROTEAN Tetra Cell Casting Module (1658021);

Mini Trans-Blot Cell (1703930);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

询价

询价






3-1

电泳-转印槽组合装

(1.5mm/10-well/2-gel/2-blot)

16580007-B

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN   Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935).

询价

询价

3-2

电泳-转印组合装

(1.5mm/10-well/4-gel/2-blot)

16580006-B

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

询价

询价

3-3

Basic电源-电泳-转印套装

(1.5mm/10-well/2-gel/2-blot)

16580007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

询价

询价

3-4

Basic电源-电泳-转印套装

(1.5mm/10-well/4-gel/2-blot)

16580006-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEA Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

询价

询价

3-5

HC电源-电泳-转印套装

(1.5mm/10-well/4-gel/4-blot)

16580006-U

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006);

Mini Trans-Blot Module (1703935);

Mini Trans-Blot Cell (1703930)

PowerPac HC Power Supply (1645052).

询价

询价






4-1

电泳-转印槽组合装

(1.0mm/10-well/2-gel)

1658003-C

Electrophoresis and blot Bundle (10-well/1.0mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658003);

Mini Trans-Blot Module (1703935).

询价

询价

4-2

Basic电源-电泳-转印套装

(1.0mm/10-well/2-gel/2-blot)

1658003-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

询价

询价

4-3

电泳-转印槽组合装

(1.5mm/10-well/2-gel)

1658007-C

Electrophoresis and blot Bundle (10-well/1.5mm/2-gel)

includes:

Mini-PROTEAN Tetra Cell system (1658007);

Mini Trans-Blot Module (1703935).

询价

询价

4-4

Basic电源-电泳-转印套装

(1.5mm/10-well/2-gel/2-blot)

1658007-S

Electrophoresis, blot and Power Supply Bundle

includes:

Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

询价

询价






5-1

蛋白预制胶

电泳-转印槽组合

1658030

Vertical Electrophoresis and Mini Trans-Blot Module Bundle

for Mini Precast 4-Gel

includes:

4-gel Mini-PROTEAN Vertical Cell (1658004);

Mini Trans-Blot Module (1703935).

询价

询价

5-2

蛋白预制胶

Basic电源电泳转印套装

1658034

Electrophoresis, blot and Power Supply Bundle

for precast 4-gel

includes:

Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004);

Mini Trans-Blot Module (1703935);

PowerPac Basic Power Supply (1645050).

询价

询价

5-3

蛋白预制胶

HC电源电泳转印套装

1658036

Electrophoresis, blot and Power Supply Bundle

includes:

for Mini Precast Gels, 4-gel

Mini-PROTEAN Tetra Cell (1658004);

Mini Trans-Blot Module (1703935);

PowerPac HC Power Supply (1645052).

询价

询价

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