小型蛋白垂直电泳槽
伯乐Mini-Protean Tetra蛋白电泳槽
伯乐Mini-Protean Tetra小型蛋白电泳槽可容纳1~4块小型手灌胶或Ready Gel 预制胶工作,用于蛋白SDS-PAGE垂直电泳分离。本栏目提供Mini-Protean Tetra的组成及选型配置方案。
设备名称:蛋白垂直电泳槽 蛋白垂直电泳仪
制 造 商:美国Bio-Rad公司 产 地:新加坡/中国
品 牌:伯乐(Bio-Rad)
型 号:Mini-Protean Tetra
电泳配件:见[主要附件] 商品价格:见[订货指南]
主要功能用途
Bio-Rad Mini- Protean Tetra蛋白垂直电泳槽,是Western Blot实验流程重要组成部件,可容纳1~4 块小型手灌胶或Ready Gel 预制胶(10×8.3cm)的电泳,主要用于蛋白聚丙烯酰胺凝胶电泳(PAGE或SDS-PAGE)实验。
性能特点
伯乐Mini-Protean Tetra蛋白垂直电泳槽,兼容Mini-Protean precast gels预制凝胶、Ready Gel precast gels预制凝胶或手工自制PAGE凝胶。
可在一小时内完成多达四块小型蛋白凝胶的电泳分离。
软橡胶垫片与玻璃板形成严密密封,确保无泄漏灌胶。
带有简单凸轮的灌制框,确保在任何平面上实现精确对齐。
平行排列的灌胶架,能同时灌制两块凝胶,弹簧杠杆设计使得软橡胶沉淀产生良好的密封性。
封边垫条的长玻板加厚,不易破碎。
标有厚度和孔数的玻板和加样梳便于识别。
上样向导防止泳道遗漏上样或重复上样。
可互换模块可将Mini- Protean Tetra电泳槽转换为可进行Western转印的 Mini Trans-Blot转印槽。
主要技术规格
伯乐Mini-Protean Tetra蛋白垂直电泳槽适用的凝胶类型包括:Mini- Protean预制胶、手灌胶
凝胶数:1–4块
凝胶格式:小型
适用的凝胶尺寸(宽×长)
预制胶:8.6×6.8cm;
手灌胶:8.3×7.3cm
玻璃板尺寸:短板10.1×7.3cm,玻板10.1×8.2cm
缓冲液运行需求
运行2块凝胶缓冲液总体积:700mL
运行4块凝胶缓冲液总体积:1000 mL
SDS-PAGE 典型运行时间:35–45 分钟(在200V恒定电压下)
Mini-PROTEAN Tetra加样梳每孔最大上样量
1.0mm 10孔梳:44 μL
1.0mm 15孔梳:26 μL
安装运行条件
相对湿度范围:0~95% R.H.
工作环境温度:0℃~40℃
外观尺寸(宽×长×高):12×16×18cm
Mini- Protean Tetra 电泳槽重量:1.0kg
(电泳电源另配)
组成配置
电泳槽系统
伯乐Mini-Protean Tetra蛋白电泳槽全套工作装置包括电泳缓冲液槽、电极组件、制胶组件和辅助工具耗材。
按一次可运行的最大凝胶数量,分为4-gel、2-gel两种规格。
如需跑4块凝胶,可订购4凝胶槽,也可在2凝胶套装基础上加配Mini-Protean Tetra相应功能附件,扩展为4块凝胶工作通量。
此外,根据使用的凝胶来源类型,还可单独配置伯乐预制胶专用套装。
品名 | 品牌 | 货号 | 规格 | 描述 | 报价 |
蛋白垂直电泳槽 (4-gel/10-well/1.0mm) | Bio-Rad | 1658001 | 1套 | Mini-PROTEAN Tetra Cell 4-gel system, 1.0mm gel thickness includes: Tank, lid with power cables Mini cell buffer dam; 1×electrode assembly; 1×companion running module(1658038); 2×casting stands, 4 casting frames; 5 sets of short plates and spacer plates; 5×10-well 1.0mm combs 5×gel releasers(1653320) | 询价 询价 |
蛋白垂直电泳槽 (4-gel/10-well/1.5mm) | Bio-Rad | 1658006 | 1套 | Mini-PROTEAN Tetra Cell 4-gel system, 1.5mm gel thickness includes: Tank, lid, power cables Mini cell buffer dam; 1×electrode assembly; 1×companion running module(1658038); 2×casting stands, 4 casting frames; 5 sets of short plates and spacer plates; 5×10-well 1.5mm combs 5×gel releasers(1653320) | 询价 询价 |
蛋白垂直电泳槽 (4-gel/10-well/0.75mm) | Bio-Rad | 1658000 | 1套 | Mini-PROTEAN Tetra Cell 4-gel system, 0.75mm gel thickness includes: Tank, lid, power cables Mini cell buffer dam; 1×electrode assembly; 1×companion running module(1658038); 2×casting stands, 4 casting frames; 5 sets of short plates and spacer plates; 5×10-well 0.75mm combs 5×gel releasers(1653320) | 询价 询价 |
蛋白垂直电泳槽l (2-gel/10-well/1.0mm) | Bio-Rad | 1658003 | 1套 | Mini-PROTEAN Tetra Cell 2-gel system, 1.0mm gel thickness includes: 1×casting stand, 2×casting frames; 5×10-well combs; 5 short plates and 5 spacer plates | 询价 询价 |
蛋白垂直电泳槽 (2-gel/10-well/1.5mm) | Bio-Rad | 1658007 | 1套 | Mini-PROTEAN Tetra Cell 2-gel system, 1.5mm gel thickness includes: 1×casting stand, 2×casting frames; 5×10-well combs; 5 sets of glass plates | 询价 询价 |
蛋白垂直电泳槽 (2-gel/10-well/0.75mm) | Bio-Rad | 1658002 | 1套 | Mini-PROTEAN Tetra Cell 2-gel system, 0.75 mm gel thickness includes: 1×casting stand, 2×casting frames; 5×10-well combs; 5 short plates and 5 spacer plates | 询价 询价 |
Mini Precast Gels(4-gel) 预制胶蛋白电泳槽 | Bio-Rad | 1658004 | 1套 | Mini-PROTEAN Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 4-gel includes: electrode assembly; companion running module; tank, lid with power cables, cell buffer dam | 询价 询价 |
Mini Precast Gels (2-gel) 预制胶蛋白电泳槽 | Bio-Rad | 1658005 | 1套 | Mini-PROTEAN Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 2-gel includes: electrode assembly; tank, lid with power cables, cell buffer dam | 询价 询价 |
电泳槽组件
伯乐Mini-Protean Tetra 小型蛋白垂直电泳槽的制胶模块(Casting Module),是指用于灌制特定厚薄和齿孔数目蛋白凝胶所需的模块化套装。
模块由通用核心组件(Core System)和差异化功能组件组成。通用核心灌胶组件为灌胶架(含4个密封垫),夹胶框(casting frames)和或胶铲(gel releaser)。差异化功能组件则包括特定规格的玻璃板套件,电泳梳和上样导槽。
伯乐Mini-PROTEAN Tetra Cell小型垂直电泳槽有多种灌胶模块可选,分别满足不同上样量和对凝胶厚度的特殊要求。
品名 | 品牌 | 货号 | 规格 | 描述 | 报价 |
1.0mm/10孔 制胶模块 | Bio-Rad | 1658015 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 10-well/1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
1.0mm/15孔制胶模块 | Bio-Rad | 1658016 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 15-well, 1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
1.0mm/5孔 制胶模块 | Bio-Rad | 1658013 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 5-well, 1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates. | 询价 询价 |
1.0mm/9孔 制胶模块 | Bio-Rad | 1658014 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 9-well, 1.0 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide. | 询价 询价 |
1.5mm/10孔 制胶模块 | Bio-Rad | 1658021 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 10-well, 1.5 mm gel casting module includes: 2×casting stands; 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
1.5mm/15孔 制胶模块 | Bio-Rad | 1658022 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 15-well, 1.5 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
1.5mm/5孔 制胶模块 | Bio-Rad | 1658019 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 5-well, 1.5 mm gel casting module includes the following componets: 2 casting stands, 4 casting frames 5 combs, 5 short plates and 5 spacer plates. | 询价 询价 |
1.5mm/9孔 制胶模块 | Bio-Rad | 1658020 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 9-well, 1.5 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
0.75mm/10孔 制胶模块 | Bio-Rad | 1658010 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 10-well, 0.75 mm gel casting module includes the following componets: 2 casting stands, 4 casting frames 5 combs, 5 short plates and 5 spacer plates sample loading guide. | 询价 询价 |
0.75mm/15孔 制胶模块 | Bio-Rad | 1658011 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 15-well, 0.75 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
0.75mm/5孔 制胶模块 | Bio-Rad | 1658008 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 5-well, 0.75 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
0.75mm/9孔 制胶模块 | Bio-Rad | 1658009 | 1套 | Mini-PROTEAN Tetra Cell Casting Module 9-well, 0.75 mm gel casting module includes: 2×casting stands, 4×casting frames; 5×combs, 5 short plates and 5 spacer plates; 1×sample loading guide | 询价 询价 |
灌胶通用套件(4胶) | Bio-Rad | 1658051 | 1套 | Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit includes: 2×stands, 4×frames,4×gaskets; 5×gel releasers. 适用于4胶Mini-Protean Tetra电泳槽 | 询价 询价 |
灌胶通用套件(2胶) | Bio-Rad | 1658052 | 1套 | Mini-PROTEAN Tetra Cell Casting Stand with Clamp Kit includes: 1×stand, 2×frames, 2×gaskets; 5×gel releasers. 适用于2胶Mini-Protean Tetra电泳槽 | 询价 询价 |
基本耗材
伯乐Mini-Protean Tetra垂直电泳槽工作的基本耗材主要包括蛋白凝胶玻璃板等。
品名 | 品牌 | 货号 | 规格 | 描述 | 报价 |
1.0mm厚玻璃板 | Bio-Rad | 1653311 | 5块/包 | Spacer Plates with 1.0 mm Integrated Spacers 带1.0mm厚封边条,为1.0mm凝胶灌胶组件。 | 询价 |
1.5mm厚玻璃板 | Bio-Rad | 1653312 | 5块/包 | spacer plate with 1.5 mm integrated spacer 带1.5mm厚封边条,用于1.5mm凝胶的制备 | 询价 |
薄玻璃板 | Bio-Rad | 1653308 | 5块/包 | short plate for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber. 外形尺寸(W×L):101× 73 mm 与带任一厚度封边垫条的长玻璃板配套使用 | 询价 |
订货指南
1、蛋白电泳槽的选型
因不同实验样品样品数量、蛋白类型和表达丰度、实验经验与操作习惯的不同,选择蛋白电泳槽重点要结合所用凝胶的厚度(0.75mm、1.0mm、1.5mm)、每张凝胶电泳的上样孔数(常用的10孔-最大上样量66μl、15孔-40μl)、每轮电泳的凝胶通量(4胶、2胶)和凝胶类型(手灌胶、预制胶)来确定具体选型方案。
如单独订购Mini-Protean Tetra小型蛋白垂直电泳槽或更换电泳灌胶配件,请请参考本栏目“组成配置”“主机”部分所列各型电泳槽的技术规格和详细配置内容。最常用的1658001槽单元,其标准配置适用的是1.0mm/10-well/4-gel凝胶。
2、蛋白电泳槽电源的选择
伯乐Mini-Protean Tetra小型蛋白垂直电泳槽本身工作运行对电源并无特殊要求。伯乐PowerPac Basic 、PowerPac HC、PowerPac Universal等多个型号均可满足同时给1-4个电泳槽供电的要求。
但须注意的是,通常情况下,蛋白电泳槽只是Western Blot实验流程的一个功能组件,单个电泳槽输出凝胶数量(2块或4块)与电转印槽(2块)各自最大凝胶工作通量不同,要充分考虑实验流程上的有效衔接问题。
特别是当实验样品数量多于4块凝胶的情况下,根据自身实验条件和经验,通盘考虑电转印槽、电转印模块的配置数量。
与常规140V左右恒压蛋白电泳跑胶不同,电转印对电流要求较高。通常,每块凝胶-印迹膜的工作电流可达200mA甚至更高。若同时进行4块凝胶转膜操作,单一Basic电源很可能无法满足供电要求。故需考虑HC、PowerPac Universal等具有电流输出能力大于0.8A的电源型号确有必要。
3、电泳槽与与Mini-Trans Blot小型蛋白电泳转印槽搭配
电转移槽的选型配置问题,请参考Mini-Trans Blot小型蛋白转印槽栏目内容。
序号 | 品名 | 货号 | 套装配置描述 | 报价 |
1-1 | Basic蛋白电泳套装 (1.0mm/10-well/4-gel) | 1658025 | Mini-PROTEAN Tetra and Power Supply Bundle includes: Mini-PROTEAN Tetra 4-gel system (1658001); PowerPac Basic power supply (1645050). | 询价 询价 |
1-2 | HC蛋白电泳套装 (1.0mm/10-well/4-gel) | 1658027 | Mini-PROTEAN Tetra and Power Supply Bundle includes: Mini-PROTEAN Tetra 4-gel system (1658001); PowerPac HC power supply (1645052). | 询价 询价 |
2-1 | 电泳-转印槽组合装 (1.0mm/10-well/4-gel/2-blot) | 1658029 | Electrophoresis and blot Bundle (10-well/1.0mm/4-gel) includes: Mini-PROTEAN Tetra Cell system (1658001); Mini Trans-Blot Module (1703935). | 询价 询价 |
2-2 | Basic电源-电泳-转印套装 (1.0mm/10-well/4-gel/2-blot) | 1658033 | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 询价 询价 |
2-3 | HC电源-电泳-转印套装 (1.0mm/10-well/4-gel/2-blot) | 1658035 | Electrophoresis, blot and Power Supply Bundle for 10-well, 1.0 mm, 4-gel system includes: Mini-PROTEAN Tetra Cell (1658001); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052) | 询价 询价 |
2-4 | HC电源-电泳-转印套装 (1.0mm/10-well/4-gel/4-blot) | 1658035-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini Trans-Blot Transfer Cell (1703930); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 询价 询价 |
2-5 | HC电源-电泳-转印高级套装 (1.0mm/10-well/4-gel) (1.5mm/10-well/4-gel) (4-blot) | 1658035-U | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658001); Mini-PROTEAN Tetra Cell Casting Module (1658021); Mini Trans-Blot Cell (1703930); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 询价 询价 |
3-1 | 电泳-转印槽组合装 (1.5mm/10-well/2-gel/2-blot) | 16580007-B | Electrophoresis and blot Bundle (10-well/1.5mm/2-gel) includes: Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007); Mini Trans-Blot Module (1703935). | 询价 询价 |
3-2 | 电泳-转印组合装 (1.5mm/10-well/4-gel/2-blot) | 16580006-B | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); | 询价 询价 |
3-3 | Basic电源-电泳-转印套装 (1.5mm/10-well/2-gel/2-blot) | 16580007-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 2-gel (1658007); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 询价 询价 |
3-4 | Basic电源-电泳-转印套装 (1.5mm/10-well/4-gel/2-blot) | 16580006-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEA Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 询价 询价 |
3-5 | HC电源-电泳-转印套装 (1.5mm/10-well/4-gel/4-blot) | 16580006-U | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658006); Mini Trans-Blot Module (1703935); Mini Trans-Blot Cell (1703930) PowerPac HC Power Supply (1645052). | 询价 询价 |
4-1 | 电泳-转印槽组合装 (1.0mm/10-well/2-gel) | 1658003-C | Electrophoresis and blot Bundle (10-well/1.0mm/2-gel) includes: Mini-PROTEAN Tetra Cell system (1658003); Mini Trans-Blot Module (1703935). | 询价 询价 |
4-2 | Basic电源-电泳-转印套装 (1.0mm/10-well/2-gel/2-blot) | 1658003-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658003); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 询价 询价 |
4-3 | 电泳-转印槽组合装 (1.5mm/10-well/2-gel) | 1658007-C | Electrophoresis and blot Bundle (10-well/1.5mm/2-gel) includes: Mini-PROTEAN Tetra Cell system (1658007); Mini Trans-Blot Module (1703935). | 询价 询价 |
4-4 | Basic电源-电泳-转印套装 (1.5mm/10-well/2-gel/2-blot) | 1658007-S | Electrophoresis, blot and Power Supply Bundle includes: Mini-PROTEAN Tetra Cell for handcast 4-gel (1658007); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 询价 询价 |
5-1 | 蛋白预制胶 电泳-转印槽组合 | 1658030 | Vertical Electrophoresis and Mini Trans-Blot Module Bundle for Mini Precast 4-Gel includes: 4-gel Mini-PROTEAN Vertical Cell (1658004); Mini Trans-Blot Module (1703935). | 询价 询价 |
5-2 | 蛋白预制胶 Basic电源电泳转印套装 | 1658034 | Electrophoresis, blot and Power Supply Bundle for precast 4-gel includes: Mini-PROTEAN Tetra Cell for Mini Precast Gels (1658004); Mini Trans-Blot Module (1703935); PowerPac Basic Power Supply (1645050). | 询价 询价 |
5-3 | 蛋白预制胶 HC电源电泳转印套装 | 1658036 | Electrophoresis, blot and Power Supply Bundle includes: for Mini Precast Gels, 4-gel Mini-PROTEAN Tetra Cell (1658004); Mini Trans-Blot Module (1703935); PowerPac HC Power Supply (1645052). | 询价 询价 |
(更多配置方案详情请咨询客服)
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