热循环模块

PCR反应模块

96-Well模块

样品通量

96×0.2 mL

样品体积

1 – 125µl（推荐10–125µl）

兼容耗材

96×0.2 mL单管

0.2mL Low-Profile Strips

Microseal Semi-Skirted Low-Profile   96-Well Plates

Multiplate Unskirted Low-Profile

温度控制

控温方式

Peltier半导体控温

热盖温度设定范围

30–110℃

最大升降温速率

2.5 ℃/sec

平均升降温速率

2.0 ℃/sec

温度控制范围

4–100 ℃

温度准确度

±0.2℃（programmed target at   90℃）

温度均匀性

±0.4℃(well-to-well after 10 sec of arrival at target)

梯度温度设定范围

30–100 ℃

温度梯度温差范围

1–24 ℃

光学检测模块

激发滤光片设置

6 个带滤光片 LED：

CH1：450-490 nm/FAM, SYBR   Green I

CH2：515-535 nm/VIC, HEX,   CAL Fluor Gold 540, Cal  Fluor Orange 560

CH3：560-590 nm/ROX, Texas   Red, CAL Fluor Red 610, TEX 615

CH4：620-650 nm/CY5, Quasar   670

CH5：672-684 nm/Quasar 705,   Cy5.5

检测滤光片设置

6 个带滤光片光敏二极管：

CH1：515-530 nm

CH2：560-580 nm

CH3：610-650 nm

CH4：675-690 nm

CH5：705-730 nm

多重分析功能

5个目标基因

FAM/SYBR Green检测时间

3秒

所有通道检测时间

12 秒

检测灵敏度

单拷贝人基因组基因

检测动态范围

10 数量级

熔解曲线分辨率

0.1 ℃

操作控制

触摸屏角度调整

旋转角度范围 12–55º可调

主板集成操作系统

Windows 10 IoT版

数据分析软件

CFX Manager Software数据分析模式：

标准曲线绝对定量

熔解曲线分析、高分辨率熔解曲线

等位基因分型

终点荧光分析

∆∆Cq 基因表达分析

最适内参筛选、带扩增效率校正的多内参基因相对定量(柱形图、散点图、火山图、聚类分析、热图)

软件操作系统

Windows 7 (64-bit), Windows 10   (64-bit);

macOS (10.14, for analysis only)

数据接口

USB 2.0 or above, Ethernet, WiFi

质量认证

IEC，CE

CFX Opus Deepwell

Real-Time PCR Instrument

Bio-Rad

12016658

Each

CFX Opus Deepwell Real-Time PCR Detection system

96-well/5-color plus FRET/network-connected

includes:

CFX Opus Deepwell base unit;

communication cables.

(Order CFX Maestro Software   separately)

询价

CFX Maestro Software (Windows)

Bio-Rad

12013758

each

CFX Maestro Software Version 2

compatible with Win 7 and 10

includes 1 license for qbase+ Software.

询价

CFX Maestro Software (Mac)

Bio-Rad

12004128

each

CFX Maestro Software Version 1.1, Mac Edition

compatible with Mac OS X El  Capitan and macOS Sierra

 (does not support instrument control)

询价

询价

Precision Melt Analysis Software

Bio-Rad

1845015

each

Precision Melt Analysis Software

includes 2 user licenses,

installation CD,

2 HASP HL keys

询价

CFX  Opus 无线网卡

Bio-Rad

12013344

each

Wi-Fi antenna enables Wi-Fi  connection

for CFX Opus in China

  询价

PCR单管盖管器

Bio-Rad

ECT1000

1个

Easy Cap Tool

ensures tight seal for 0.2mL PCR tube/96-well microplate

询价

PCR排管盖管器

Bio-Rad

ECT2000

1个

Strip Cap Tool

for sealing 8- and 12-cap strips on PCR plates or tubes

询价

彩色PCR样品管座

Bio-Rad

TRC0501

5个/包

96-Place Racks with covers, assorted colors

Application for：

PCR tubes；

unskirted and semi-skirted microplates

询价

ANSI/SBS标准PCR样品管座

Bio-Rad

TRC9601

10个/包

96-place rack

ANSI/SBS standards, autoclavable, white

holds 0.2 and 0.1 ml PCR tubes and plates

询价

密封膜滚筒

Bio-Rad

MSR0001

1个

Film Sealing Roller for PCR Plates

Sealing Roller for sealing PCR plates with films

询价

硬框96孔定量PCR板

Bio-Rad

HSL9605

25块/盒

Hard-Shell 96-Well PCR Plates

low profile, semi-skirted

clear shell/white well PCR plate

询价

硬框96孔定量PCR板

（绿边-白孔）

Bio-Rad

HSL9645

25块/盒

Hard-Shell 96-Well PCR Plates

low profile, semi-skirted

green shell/white well PCR plate

（discontinued）

询价

编码硬框96孔定量PCR板

Bio-Rad

HSL9905

25块/盒

Hard-Shell 96-Well PCR Plates

low profile, semi-skirted, barcoded

clear shell/white well PCR plate

barcoded on 2 sides (Row H and Column 12)

（discontinued）

询价

定量PCR封板膜B

Bio-Rad

MSB1001

100张/包

Microseal B Optical Adhesive PCR Plate Sealing Film

询价

定量PCR封板膜C

Bio-Rad

MSC1001

100张/包

Microseal C Optical Adhesive PCR Plate Sealing Film

询价

白色定量PCR八联管

Bio-Rad

TLS0851

120条/盒

0.2ml 8-Tube PCR Strips without Caps

low profile, white, thin-wall

for PCR and low-volume reactions

询价

透明PCR   8联管

Bio-Rad

TLS0801

120条/盒

0.2ml 8-Tube PCR Strips without Caps

low profile, clear, thin-wall

for PCR and low-volume reactions

询价

定量PCR   八联平盖

Bio-Rad

TCS0803

120条/盒

8-cap strips for 0.2ml tubes and plates

for PCR and optical reactions, ultraclear

询价

cDNA合成高级试剂盒

Bio-Rad

1725037

个

iScript Advanced  cDNA Synthesis Kit

25 x 20 µl reactions

includes：

100µl iScript Advanced 5x Reaction Mix

25µl iScript Advanced Reverse Transcriptase

询价

cDNA合成预混液

Bio-Rad

1708840

个

iScript Reverse Transcription Supermix

25 x 20 µl reactions, 100µl

询价

抑制剂耐受型高级SYBR   Green预混液

Bio-Rad

1725016

个

SsoAdvanced Universal Inhibitor-Tolerant

SYBR Green Supermix

200 x 20 µl rxns, 2 ml (2 x 1 ml)

询价

SYBR Green通用预混液

Bio-Rad

1725150

个

iTaq Universal SYBR Green One-Step Kit

100 x 20 µl   reactions, 1 ml

询价

SYBR Green通用预混液

Bio-Rad

1725120

个

iTaq Universal SYBR   Green Supermix

200 x 20µl   reactions, 2 ml (2 x 1ml)

询价

快速EvaGreen qPCR预混液（ROX）

Bio-Rad

1725210

个

SsoFast EvaGreen Supermix with Low ROX

200 x 20 µl rxns, 2ml (2 x 1.0ml)

询价

快速EvaGreen qPCR预混液

Bio-Rad

1725200

个

SsoFast EvaGreen Supermix

2x real-time PCR mix

for 200 x 20 µl reactions，2×1ml

询价

探针通用预混液

Bio-Rad

1725140

个

iTaq Universal Probes One-Step Kit

100 x 20µl   reactions, 1ml

询价

探针通用预混液

Bio-Rad

1725130

个

iTaq Universal Probes Supermix

200 x 20µl   reactions, 2ml (2 x 1ml)

询价

高级探针预混液

Bio-Rad

1725280

个

SsoAdvanced Universal Probes Supermix

200 x 20 µl rxns, 2 ml (2 x 1 ml)

询价

高级预混液

Bio-Rad

1725160

个

SsoAdvanced PreAmp Supermix

50×50µl rxns, 1.25ml (1×1.25ml)

询价

多重定量PCR预混液

Bio-Rad

1725848

个

iQ Multiplex Powermix, 1.25ml

50 x 50 µl  reactions

询价

多重定量PCR用预混液

Bio-Rad

12010176

EA

Reliance One-Step Multiplex RT-qPCR Supermix

200 x 20 µl reactions

询价

1

CFX Opus Deepwell

荧光定量PCR仪入门套装

17008093

CFX Opus Deepwell Real-Time PCR System with   Starter Package

includes:

CFX Opus Deepwell Real-Time PCR Instrument (12016658);

CFX Maestro Software for Windows;

license for qbase+ Software;

consumables

询价

2

CFX Opus Deepwell

荧光定量PCR仪标准套装

17008093-S

CFX Opus Deepwell Real-Time PCR System Research Package

includes:

CFX Opus Deepwell Real-Time PCR System with Starter Packag (17008093)

2）PCR体系构建工具套件，含：

含PCR单管盖管器(ECT1000);

PCR排管盖管器(ECT2000);

PCR板封膜滚筒(MSR0001);

96孔彩色PCR管架(TRC0501)

3）外置电脑工作站（windows）

4）CFX  Opus Wi-Fi   Adapter (China)

询价